Alexa,fluor,700,mouse,antihuman,cd14,m5e2,ruo 561029 bd. Jun 10, 2012 cyanine dyes are comparatively old fluorescent dyes and the basis for other fluorochromes with improved brightness, photostability, quantum yield etc. Apc alexa fluor 700 could be a better one, but the products are rather limited. The alexa fluor dyes except for alexa fluor 430, pacific blue dye, and cascade blue dye are covered by pending and issued patents.
The alexa fluor 660 dye is efficiently excited by the 633 nm and 647 nm spectral lines of the hene laser and the kryptonion laser, respectively. Alexa fluor 488 fitc 488 nm cy5 651 nm alexa fluor 647 wavelength nm 400 700 nm infrared fluorophores 700 nm uvvisible fluorophores popular applications. Alexa fluor 700 emits light at 775 nm, and has a molar mass of approximately 0 gmol. However, these commercial proteinlabeling cydyes and alexa fluor.
Fluoronanogold combined fluorescent and gold nanoparticle. These postsynthesis manually coupled dyes are available in a wide spectral range from blue to deep red. Properties and structures a range of alexa dyes is available, with emission wavelengths spanning the visible spectrum and extending into the infrared region of the spectrum. Excitation and emission spectral profiles energy level. Each tide fluor dye is developed to match the spectral properties of a particular alexa fluor or other labeling dyes such as dylight dyes. Laser 1 quantum brightness fluorochrome em nm filter lp. Alexa fluor dyes are generally more stable, brighter, and less phsensitive than common dyes e. Handbook of fluorescent dyes and probes is the most comprehensive volume available on the subject, covering all the available dyes and probes known to date in the literature for uses in. Biolegend provides a convenient fluorophore brightness index as a tool for multicolor flow cytometry. The fluorescence quantum yield of hilyte fluor 594 is 0. Alexa fluor 700 696 719 molecular probes alexa fluor 750 752 779 molecular probes cy2 489 506 714 ap biotech. They are the best affordable fluorescent dyes alternative to alexa fluor dyes for labeling oligos and peptides without comprised performance.
Alexa fluor 700 antihuman cd4 antibody anticd4 okt4. When working in this scope, biological samples, blotting membranes, plastics and chemical compound libraries for highthroughput. Quantitative comparison of longwavelength alexa fluor. Substance name solvent ph excitation max nm emission max nm qdot 705. The alexa fluor dyes, and other dyes that emit at longer wavelengths, towards the red end of the spectrum 600 nm have advantages over dyes that emit at shorter wavelengths in that there is typically less background fluorescence at longer these wavelengths. Sulfonation makes alexa fluor dyes negatively charged and hydrophilic. Alexa fluor 700 antimouse cd8a antibody anticd8a 536. Fluorochromes description cincinnati childrens hospital.
The chapter also includes references on the use and properties of alexa fluor 700. For tandem conjugates, human peripheral blood was collected in a vacutainer tube as above and centrifuged at 3300. Properties and structures a range of alexa dyes is available, with emission. There are currently no images for il4 antibody ic6543n. Cytometry alexa fluor 700, apcalexa fluor 700 and efluor 710. Before staining cells with this reagent, please confirm that your flow cytometer is capable of exciting the fluorochrome and discriminating the resulting fluorescence. Alexa fluor fluoronanogold is a unique, dualpurpose probe.
Used for stable signal generation in imaging and flow cytometry, alexa fluor 700 dye is water soluble and phinsensitive from ph 4 to ph 10. Table of fluorochromes salk institute for biological studies. When conjugated to proteins, cf594 is significantly brighter than alexa fluor 594 figure 2 due to its high quantum yield and exceptional water solubility. Quantitative comparison of longwavelength alexa fluor dyes. Alexa fluor 700 carboxylic acid succinimidyl ester handbook. Last class fluorescence absorption emission jablonski diagrams this class more on fluorescence common fluorophores. Other properties listed for this fluorescent dye include the physical form, solubility, absorption maxima, emission maxima, molar extinction coefficient and quantum yield. Alexa fluor 700 is a farred dye that can be used in. Coeff e quantum yield qy brightness exqy100 commentsuse. When a fluorophore absorbs a photon of light, an energetically excited state is formed. Alexa 350, alexa 430, alexa 488, alexa 532, alexa 546, alexa 568, and alexa 594 dyes are a new series of fluorescent dyes with emissionexcitation spectra similar to those of amca, lucifer yellow, fluorescein, rhodamine 6g, tetramethylrhodamine or cy3, lissamine rhodamine b, and texas red, respectively the numbers in the alexa. Prior to using alexa fluor 700 conjugate for flow cytometric analysis, please verify your flow cytometers capability of exciting and detecting the fluorochrome. Novel rhodamine chemistry rhodamine dyes are known for their excellent photostability and good fluorescence quantum yield. Alexa fluor 700 is a bright and photostable nearir dye that can be excited with a xenonarc lamp, farred diode lasers, or dye pumped lasers operating in the 675 700 nm range.
The alexa, or alexa fluor, dyes were introduced by molecular probes, inc. Flow cytometry was also used to compare a cy7 apc sa tandem conjugate to apc sa conjugates of alexa fluor 680, alexa fluor 700, or alexa fluor 750 dye. They are available in a wide spectral range from blue to deep red. Quantitative comparison of longwavelength alexa fluor dyes to cy dyes. Cyanine dyes are comparatively old fluorescent dyes and the basis for other fluorochromes with improved brightness, photostability, quantum yield etc.
They are conjugated postsynthesis to an aminomodified oligo as nhs esters. With the wide range of invitrogen alexa fluor dyes there is something for every filter and many options for multiplex detection. The chapter also includes references on the use and properties of alexa fluor 700 carboxylic acid succinimidyl ester. Within groups, roughly in order of excitation wavelength families excepted. Ram sabnis organizes the compounds alphabetically by the most commonly used chemical name. The dyes and protein conjugates of the dyes were compared spectrally, and the functionality of dyeprotein conjugates was examined in several applications.
Higher ozone stability than alexa fluor 647 or cy5. Alexa dyes, a series of new fluorescent dyes that yield. Hilyte fluor 594 conjugated streptavidin provides high fluorescence intensity and. The fluorophore brightness index score is a relative indication of fluorescence. Alexa fluor 700 696 719 molecular probes alexa fluor 750 752 779. Within groups, roughly in order of excitation wavelength. Alexa fluor 555 cy3 ethidium bromide ethidium homodimer1 propidium iodide resorufin rfp rhod2. Fluorochrome ex nm em nm filter lp ecm m quantum yield. New rhodamine chemistry rhodamine dyes are known for their excellent photostability and good fluorescence quantum yield. In contrast to alexa fluor 647, the low photon yield of atto 655. Alexa fluor 700 carboxylic acid succinimidyl ester. Evaluation of fluorophores for optimal performance in. Every product we sell is backed by novus 100% guarantee.
Amca conjugates, the fluorescence of alexa fluor 350 conjugates is better separated from that of. Hilyte fluor dyes spanning the full visible spectrum. Color bars are an approximation of corresponding emission wavelength. Tide fluor dyes for fluorescence labeling and fret. A lower yield may result from polymodifications andor strong secondary structures minimum guaranteed yields. Alexa fluor 700 is a molecular probe exhibiting a red color which absorbs light at 702 nm.
Alexa fluor 700 has an adsorption maximum of 700nm and a peak fluorescence emission of 720nm. For alexa fluor 660, alexa fluor 680, alexa fluor 700. Alexa fluor dyes sulfonation of coumarin rhodamine cyanine dyes. The absorption maximum of alexa fluor 546 occurs near 556 nm, and the emission maximum is near 573 nm. Fret acceptors with low quantum yield may provide very low dynamic. This is a table of some characteristics of fluorochromes useful for flow cytometry or fluorescence microscopy. Probes with high fluorescence quantum yield and high photostability allow detection of lowabundance biological structures with great sensitivity. The alexa fluor 700 emits at 723 nm and can be excited with the red laser 633 mm. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. The alexa fluor 660 dye exhibits good spectral separation from orange fluorophores, including the alexa fluor 555 dye and phycoerythrin pe, making it a great choice for multicolor applications. Tide fluor dyes for fluorescence labeling and fret applications.
For alexa fluor 660, alexa fluor 680, alexa fluor 700 and alexa fluor 750 dyes, qy measurements were made in pbs 50 mm potassium phosphate, 150 mm nacl, ph 7. Alexa dyes, a series of new fluorescent dyes that yield exceptionally bright, photostable conjugates. Cf594 also has excellent photostability figure 3, making it ideal for demanding. Name max absorption max emission extinction coefficient quantum yield brightness index conditions. Human vision is insensitive to light beyond 650 nm, and therefore it is not possible to view the farredfluorescent dyes by looking.
Fluorescent imaging systems commonly operate in the visible wavelength range between 400 and 700 nm. Alexa fluor700 antibodies are conjugated to alexa fluor 700. Alexa fluor, pacific blue, and pacific orange antibody. Alexa fluor is a trademark of molecular probes inc. The alexa fluor family of fluorescent dyes is a series of dyes invented by molecular probes, now a part of thermo fisher scientific, and sold under the invitrogen brand name. Alexa fluor fluoronanogold contains an extremely uniform 1. Spans the nearuv, visible and nearir spectrum figure 1, table 1. Alexa fluor dyes antibody conjugates molecular probes. Fluorophores guide uv kit dapi ebfp hoechst 33258 dye 7hydroxy4methylcoumarin quinine sulfate. Alexa fluor594 fluoronanogoldstreptavidin consists of streptavidin conjugated to alexa fluor 594 dye red and the 1. For alexa fluor 660, alexa fluor 680, alexa fluor 700 and alexa fluor 750 dyes, qy.
I have contacted invitrogenmolecular probes and got the answer that the streptavidin apc alexa fluor 700 is stopped for selling reasons not clear. Human vision is insensitive to light beyond 650 nm, and therefore it is not possible to. Handbook of fluorescent dyes and probes is the most comprehensive volume available on the subject, covering all the available dyes and probes known to date in the literature for uses in various fields. Quantitative comparison of longwavelength alexa fluor dyes to. In vivo imaging maximize the depth of tissue penetration using our comprehensive selection of infrared fluorescent in vivo imaging reagents. A extinction coefficient of the acceptor m1 cm1 qy a quantum yield of the acceptor.
Alexa fluor 647 dye is a bright, farredfluorescent dye with excitation ideally suited for the 594 nm or 633 nm laser lines. Reference standard for measuring fluorescence quantum yield. Alexa fluor dyes are frequently used as cell and tissue labels in fluorescence microscopy and cell biology. Alexa dyes have high quantum yield and are relatively photostable. A 100% efficient fluorescence process would have a quantum yield of 1 the maximum quantum yield possible.
Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the apc and alexa fluor 700 detectors. I have worked a little before with alexa fluor 700, but it seems to be a weak dye. Alexa 350, alexa 430, alexa 488, alexa 532, alexa 546, alexa 568, and alexa 594 dyes are a new series of fluorescent dyes with emissionexcitation spectra similar to those of amca, lucifer yellow, fluorescein, rhodamine 6g, tetramethylrhodamine or cy3, lissamine rhodamine b, and texas red, respectively the numbers in the alexa names indicate the approximate excitation wavelength maximum in nm. Laser 1 quantum brightness fluorochrome em nm filter lp e.
Alexa fluor 700 has a maximum emission of 719 nm when it is excited at 633nm 635nm. Alexa fluor 700 antihuman cd4 antibody cd4, also known as t4, is a 55 kd singlechain type i transmembrane glycoprotein expressed on most thymocytes, a subset of t cells, and monocytesmacrophages. By combining gold and fluorescence into one immunoprobe, the same specimen may be imaged using both fluorescence microscopy e. Fluorochromefluorescent proteinprobeindicator ex nm em nm mw oligomeric state mol. A guide to recording fluorescence quantum yields introduction. For alexa fluor 660, alexa fluor 680, alexa fluor 700 and. The quantum yield is a readout of the efficiency of the fluorescence process. Alexa,fluor,700,mouse,antihuman,cd3,ucht1,also,known,as.
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